ABSTRACT
Phytoestrogens are known to have beneficial properties in various carcinomas. They exhibit its efficacy at cellular levels. Naringenin a flavonoidal phytoestrogen is been explored for its antioxidant, cardio protective and cytotoxic function. The low absorbtion and poor bioavailability of naringenin makes it less efficient in targeting tumours at cellular levels. Due to the structural similarity of naringenin with estradiol and considering the affinity of naringenin with estrogen receptor, this study explores the interactions of naringenin on important signaling proteins involved in ER positive breast cancer through molecular docking studies and the prepared naringenin solid lipid nano particles were characterized and studied for its preventive potential against breast cancer cell lines. The lipidoid form of phytoestrogen shows promising cytotoxic potential compared with naringenin.
ABSTRACT
One of the most common causes of mortality in COVID-19 patients is cytokine release syndrome (CRS). Though several cytokines are involved in CRS, the role of Interleukin 6 is significant. Considering the importance of IL-6 inhibition and the drawbacks of the existing monoclonal antibodies, the present study develops new flavonoid metal complexes as immune boosters targeting IL-6 for SARS-CoV-2 treatment. To identify the potential flavonoids from 152 secondary plant metabolites, PyRx 0.9 tool has been used. The top scorer quercetin was converted into quercetin-oxime. Seven metal complexes (QM-1 to QM-7) were made from quercetin-oxime by utilizing divalent metals such as zinc, copper, magnesium, cobalt, barium, and cadmium. It was assumed that all compounds were moderately soluble and would not penetrate the BBB through in silico ADME studies. However, the in vitro heamolytic research revealed a modest heamolytic effect in all seven complexes. To know the IL-6 inhibitory potential preliminary level, the complexes were screened for cytotoxicity in cell lines MCF-7 which predominantly expresses the IL-6 level. The cytotoxic effects of all complexes were considerable relative to the marketable Nutridac formulation. The complexes quercetin-Zinc (QM1) and quercetin-Zinc-Ascorbic acid (QM7) showed significant cytotoxicity on MCF-7 compared to Nutridac and no cytotoxic toward the normal cell lines.
ABSTRACT
The present study focuses on the role of remote sensing and geographic information system (GIS) in assessment of changes in forest cover, between 1931 and 2001, in the Kalrayan hills, Tamil Nadu. The trend of forest cover changes over the time span of 70 years, was precisely analysed using high resolution Satellite data. The study revealed that the forest cover was 275.6, 481.7 and 266.5 sq.km in 1931, 1971 and 2001 respectively. It was noticed that forest cover has increased between 1931 and 1971, because of the implementation of various afforestation schemes by the forest department and scared grooves. It also revealed that the forest cover loss between 1971 and 2001 could be due to Shifting cultivation and illegal encroachments by villagers; and the forest cover drastically decreased on plateau areas due to human population pressure. The study analyses the forest cover change in the tropical deciduous forest region of the Eastern Ghats of India. It is envisaged that the study would prove the usefulness of Remote Sensing and GIS in forest restoration planning.